rabbit polyclonal anti lc3 i ii (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit polyclonal anti lc3 i ii
Rabbit Polyclonal Anti Lc3 I Ii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+lc3+i+ii/pmc13052180-62-119-124
Average 86 stars, based on 1 article reviews

rabbit polyclonal anti lc3 i ii - by Bioz Stars, 2026-09

86/100 stars

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Article Title: 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells
Article Snippet: Primary antibodies used were: rabbit polyclonal anti-HADHA (Proteintech, Cat# 10758-1-ap) 1:500; rabbit polyclonal anti-SQSTM1/p62 (Novus, Cat# NBP-48320, Centennial, CO, USA) 1:4000; mouse monoclonal anti-phosphoERK1/2 (Santa Cruz, Cat# 7383, Dallas, TX, USA) 1:500; rabbit polyclonal anti-ERK1 and anti-ERK2 (Santa Cruz, Cat# sc-93 and Cat# sc-154) 1:500; rabbit polyclonal anti-p21 (Proteintech, Cat# 10355-1-AP) 1:800; rabbit polyclonal anti-LC3 I/II (Novus, Cat# NB100-2220) 1:1000; rabbit monoclonal anti-phospho4EBP1 (Cell Signaling Technology, Cat# 2855T, Danvers, MA, USA) 1:500; mouse monoclonal anti-4EBP1 (Proteintech, Cat# 60246-1-1g) 1:500; mouse monoclonal anti-β Actina (Sigma Aldrich, Cat# A5441) 1:10,000.

Article Title: Autophagy-mediated HMGB1 release antagonizes apoptosis of gastric cancer cells induced by vincristine via transcriptional regulation of Mcl-1.
Article Snippet: Autophagy-mediated HMGB1 release antagonizes apoptosis of gastric cancer cells induced by vincristine via transcriptional regulation of Mcl-1 ZhenZhen Zhan, Qun Li, Ping Wu, Yan Ye, Hsin-Yi Tseng, Linjie Zhang & Xu Dong Zhang To cite this article: ZhenZhen Zhan, Qun Li, Ping Wu, Yan Ye, Hsin-Yi Tseng, Linjie Zhang & Xu Dong Zhang (2012) Autophagy-mediated HMGB1 release antagonizes apoptosis of gastric cancer cells induced by vincristine via transcriptional regulation of Mcl-1, Autophagy, 8:1, 109-121, DOI: 10.4161/auto.8.1.18319 To link to this article: https://doi.org/10.4161/auto.8.1.18319

Article Title: Autophagy-mediated HMGB1 release antagonizes apoptosis of gastric cancer cells induced by vincristine via transcriptional regulation of Mcl-1.
Article Snippet: Antibodies were obtained from the following sources: rabbit polyclonal anti-TLR2 (Abcam, ab24192), mouse monoclonal anti-TLR4 (Abcam, ab22048), mouse monoclonal anti-RAGE (Abcam, ab54741), rabbit polyclonal anti-LC3-I/II (Cell Signaling Technology, 4108), rabbit monoclonal anti-Beclin 1(Cell Signaling Technology, 3495), rabbit monoclonal anti-Atg5 (Cell Signaling Technology, 8540), rabbit monoclonal anti-p62 (Cell Signaling Technology, 8025), rabbit monoclonal anti-cleaved Caspase-3 (Cell Signaling Technology, 9664), rabbit monoclonal anti-Mcl-1 (Cell Signaling Technology, 5453), rabbit monoclonal anti-Bcl-2 (Cell Signaling Technology, 2870), rabbit monoclonal anti-Bcl-xL (Cell Signaling Technology, 2764), rabbit monoclonal anti-cleaved PARP (Cell Signaling Technology, 5625), rabbit polyclonal antiHMGB1 antibody (Sigma, H-9664), mouse monoclonal antiubiquitin (Sigma, U0508), mouse monoclonal anti-β-actin (Santa Cruz, sc-81178), mouse monoclonal anti-cytochrome c (Santa Cruz, sc-13156).

Western Blot:

Article Title: A heterozygous p.S143P mutation in LMNA associates with proteasome dysfunction and enhanced autophagy-mediated degradation of mutant lamins A and C.
Article Snippet: Cells were treated with 300 μg/ml cycloheximide (CHX) (Sigma-Aldrich, St. Louis, Missouri, United States), 50 μM leptomycin B (LMB) (Santa Cruz Biotechnology, Dallas, Texas, United States), 20 mM ammonium chloride (NH4Cl), 1 μM MG132 (Sigma-Aldrich) and/or 5 mM 4-Phenylbutyric acid (4-PBA) (Selleck Chemicals, Houston, Texas, United States). .. The primary antibodies included mouse monoclonal anti-lamins A and C (1:10,000, a kind gift from Professor Robert Goldman, Northwestern University), mouse monoclonal anti-actin (1:500, clone AC-40, Sigma-Aldrich), rabbit monoclonal anti-Atg5 (1:100, clone D5F5U, #12994, Cell Signaling Technology, Danvers, Massachusetts, United States), rabbit monoclonal anti-Atg7 (1:100, clone D12B11, #8558, Cell Signaling Technology), rabbit polyclonal anti-LC3-I/II (IF:1:100, WB:1:1000, #4108, Cell Signaling Technology), mouse monoclonal anti-p62 (IF:1:100, WB:1: 1000, clone 2C11, ab56416, Abcam Cambridge, United Kingdom), mouse monoclonal HRP-conjugated antiGAPDH (1:10,000, clone 1E6D9, #HRP-60004, Proteintech, Rosemont, Illinois, United States), rabbit monoclonal antiubiquitin-K48 (1:400, clone Apu2, #ZRB2150, Sigma-Aldrich) and mouse monoclonal vimentin (1:100, clone V6, #V6630, Sigma-Aldrich). .. Secondary antibodies for western blotting were HRP-conjugated donkey anti-rabbit-IgG and sheep anti- mouse-IgG (both from Thermo Fischer Scientific, Waltham, Massachusetts, United States).

Article Title: A heterozygous p.S143P mutation in LMNA associates with proteasome dysfunction and enhanced autophagy-mediated degradation of mutant lamins A and C
Article Snippet: Cells were treated with 300 μg/ml cycloheximide (CHX) (Sigma-Aldrich, St. Louis, Missouri, United States), 50 μM leptomycin B (LMB) (Santa Cruz Biotechnology, Dallas, Texas, United States), 20 mM ammonium chloride (NH 4 Cl), 1 μM MG132 (Sigma-Aldrich) and/or 5 mM 4-Phenylbutyric acid (4-PBA) (Selleck Chemicals, Houston, Texas, United States). .. The primary antibodies included mouse monoclonal anti-lamins A and C (1:10,000, a kind gift from Professor Robert Goldman, Northwestern University), mouse monoclonal anti-actin (1:500, clone AC-40, Sigma-Aldrich), rabbit monoclonal anti-Atg5 (1:100, clone D5F5U, #12994, Cell Signaling Technology, Danvers, Massachusetts, United States), rabbit monoclonal anti-Atg7 (1:100, clone D12B11, #8558, Cell Signaling Technology), rabbit polyclonal anti-LC3-I/II (IF:1:100, WB:1:1000, #4108, Cell Signaling Technology), mouse monoclonal anti-p62 (IF:1:100, WB:1:1000, clone 2C11, ab56416, Abcam Cambridge, United Kingdom), mouse monoclonal HRP-conjugated anti-GAPDH (1:10,000, clone 1E6D9, #HRP-60004, Proteintech, Rosemont, Illinois, United States), rabbit monoclonal anti-ubiquitin-K48 (1:400, clone Apu2, #ZRB2150, Sigma-Aldrich) and mouse monoclonal vimentin (1:100, clone V6, #V6630, Sigma-Aldrich). .. Secondary antibodies for western blotting were HRP-conjugated donkey anti-rabbit-IgG and sheep anti-mouse-IgG (both from Thermo Fischer Scientific, Waltham, Massachusetts, United States).

Immunofluorescence:

Article Title: Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis
Article Snippet: For the IL6-neutralizing experiment, we purchased anti-mIL6 rat monoclonal IgG (MAB406) and control rat monoclonal IgG from R&D System (MAB005). .. A horseradish peroxidase–conjugated secondary antibody (7074) and rabbit monoclonal anti-CDH5 (2500) (immunofluorescence of human cells), rabbit polyclonal anti-p-MAPK/ERK (9102), rabbit polyclonal anti-MAPK/ERK (9101), rabbit monoclonal anti-ATG14 (96752), rabbit monoclonal anti- SNAI1/SNAIL (3879), rabbit polyclonal anti-LC3-I/II (2775), and rabbit polyclonal anti-SMAD3 (9513) antibodies were purchased from Cell Signaling Technology. .. Rabbit polyclonal anti-ATG12–ATG5 complex (PM050) and rabbit polyclonal anti-SQSTM1 (PM045) antibodies were purchased from MBL.

Incubation:

Article Title: CpG oligodeoxynucleotide reduces PrP Sc accumulation and prolongs survival in prion-infected mice
Article Snippet: Equal amounts of protein (40 μg/lane) were subjected to 12 or 15% SDS-PAGE gels and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA) using an electrotransfer system (Bio-Rad Laboratories, Hercules, CA, USA). .. Membranes were blocked with 5% nonfat dry milk in PBST (8 mM Na 2 HPO 4 , 2 mM KH 2 PO4, 138 mM NaCl, 2.7 mM KCl, 0.1% Tween 20; pH 7.4) for 1 hour at room temperature (RT), followed by overnight incubation at 4 °C with the following primary antibodies: mouse monoclonal anti-PrP 3F10 (1:2000) , rabbit polyclonal anti-TLR9 (1:2000, Abcam, Cambridge, UK), rabbit polyclonal anti-phospho AMPK T172 (p-AMPK T172) (1:2000, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-AMPK (1:2000, Cell Signaling Technology), rabbit polyclonal anti-phospho-p62 S403 (p-p62 S403) (1:2000, Cell Signaling Technology), rabbit monoclonal anti-p62 (1:2000, MBL, Nagoya, Japan), rabbit monoclonal anti-phospho-ULK1 S555 (p-ULK1 S555)(1:2000, Cell Signaling Technology), rabbit polyclonal anti-ULK1 (1:2000, Cell Signaling Technology), rabbit polyclonal anti-LC3 I/II (1:2000, Cell Signaling Technology), rabbit polyclonal anti-GFAP (1:2000, CosmoBio, Tokyo, Japan), rabbit polyclonal anti-ATG12 (1:2000, Cell Signaling Technology) and mouse monoclonal anti-β-actin (1:2000, Sigma-Aldrich). .. Membranes were washed 3 times with PBST (10 minutes each) and then incubated for 1 hour at RT with the following horseradish peroxidase-conjugated secondary antibodies: goat anti-mouse IgG or goat anti-rabbit IgG (both 1:5000, Enzo Life Sciences, Farmingdale, NY, USA).

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